5,7-Dichlorolichexanthone

Background: Fine particulate matter (PM2.5) exposure is proved to be associated with illnesses, but the mechanism is not clear. Potential effects of PM2.5 on innate immunity have become a hotspot recently. Confronting PM2.5, macrophages are able to be activated and induce inflammatory responses. Whether PM2.5 exposure affects macrophage polarization and associated mechanisms remains to be further explored. Afterwards, whether Sirtuin1 (SIRT1) an important intermediate regulator in various physiological processes takes part in the macrophage polarization induced by PM2.5 is unknown. MiRNAs are acknowledged as key regulator in posttranscriptional modification and our previous study found that miR-146a is a novel biomarker of PM2.5 exposure. Thus, we propose a hypothesis, PM2.5 exposure induces M1 polarization and miR-146a-3p is a potential upstream regulator by targeting SIRT1. Methods: RAW264.7 cells were treated with different concentrations of PM2.5 for 24h. The expressions of cytokines and key molecular markers were detected by qRT-PCR, Western blotting and ELISA. The activation degree of TLRs and NF-κB was assessed by Western blotting. The specific agonist and antagonist of SIRT1 were used to explore the potential role of SIRT1 in M1 polarization induced by PM2.5. MiR-146a-3p mimic and inhibitor were pre-transfected into RAW264.7 cells and the effects on M1 polarization induced by PM2.5 were evaluated. Luciferase analysis was used to identify the binding site of miR-146a-3p and SIRT1. Results: PM2.5 increased the mRNA and protein expression of M1 markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. The protein level of TLR4 was significantly increased and the ratio of phosphorylated NF-κB p65 versus p65 subunit was also elevated in PM2.5 group. PM2.5 decreased the protein level of SIRT1 but not the mRNA expression in vitro and in vivo experiments. Pre-treatment with SIRT1 agonist SRT1720 rescued the PM2.5 induced M1 response. Whereas, SIRT1 antagonist EX527 augment the effect. MiR-146a-3p was upregulated in PM2.5 treated RAW264.7 cells. Luciferase experiments reported that SIRT1 was directly targeted by miR-146a-3p. Overexpression of miR-146a-3p downregulated the expression of SIRT1 protein in untreated RAW264.7 cells. Importantly, inhibition of miR-146a-3p upregulated SIRT1 protein and suppressed M1 polarization in PM2.5 treated RAW264.7 cells. Conclusions: These results suggested that PM2.5 induces the inflammatory M1 polarization and TLR4/NF-κB signal transduction pathway might be involved in the process. MiR-146a-3p is a novel regulator of PM2.5 exerted M1 polarization by targeting SIRT1.

Chlorine-rich argyrodite-type solid electrolyte Li5.5PS4.5Cl1.5 has been a promising choice for solid-state batteries (SSBs) because of its ultrafast Li-ion conduction. However, the poor air/moisture stability and low electrochemical stability with pristine high-voltage cathodes hinder their applications. Herein, O-substituted Li5.5PS4.5-xOxCl1.5 (x = 0, 0.075, 0.175, and 0.25) solid electrolytes are successfully synthesized. Among them, Li5.5PS4.425O0.075Cl1.5 delivers high ionic conductivity, improved moisture resistance, and enhanced electrochemical stability in higher voltage windows. SSBs using Li5.5PS4.425O0.075Cl1.5 show higher capacities and superior cyclability than those using Li5.5PS4.5Cl1.5 combined with a pristine LiNi0.8Mn0.1Co0.1O2 cathode when operated at a high end-of-charge voltage of 4.5 V (vs Li+/Li0). Moreover, the batteries exhibit outstanding performance in a wide temperature range. This work provides a strategy to modify the inherent drawbacks of sulfide electrolytes, promoting their practical applications.

The noncentrosymmetric Th7Co2.5 57Fe0.5 and Th7Ni2.5 57Fe0.5 superconductors were studied by means of 57Fe transmission Mössbauer spectroscopy (TMS), ac-magnetic susceptibility and magnetization measurements. The low-temperature data showed that both compounds are type-II superconductors with transition temperature close to [Formula: see text] K. The collected Mössbauer spectra of the studied samples consist of one well-resolved doublet over the temperature range measured. Neither abnormal behaviour of the hyperfine parameters at or near [Formula: see text] nor phonon softening were found. A comparison of 57Fe Mössbauer data of Ni- and Co-based superconductors with those of Th7Fe3 indicated that with decreasing 3d-electron numbers across the series Th7Ni2.5 57Fe0.5 [Formula: see text] Th7Co2.5 57Fe0.5 [Formula: see text] Th7Fe3, the s-electron density around the Fe nucleus and as well as the electric field gradient strength increases. The latter finding is associated with increasing asymmetricity of 3d-electron distribution and this fact presumably gives arise to an exotic superconductivity in the Co- and Fe-based compounds.