![alpha-Zearalenol-[d5]](/upload/banner/navi-3853.jpg)
alpha-Zearalenol-[d5]
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![alpha-Zearalenol-[d5]](https://resource.bocsci.com/structure/36455-72-8.gif)
| Category | Mycotoxins |
| Catalog number | BBF-04601 |
| CAS | 36455-72-8 |
| Molecular Weight | 325.41 |
| Molecular Formula | C18H19D5O5 |
| Purity | 95% by HPLC; 98% atom D |
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Description
α-Zearalenol-[d5] is a labelled impurity of Zearalenol. Zearalenol, a resorcylic acid lactones derivative, could be produced by Fusarium fungi in grains and has been found to be an environmental contaminant.
Specification
| Related CAS | 36455-72-8 (unlabelled) |
| Synonyms | α-Zearalenol-d5; (E)-(7S,11R)-2,4,11-Trihydroxy-7-methyl-7,8,9,10,11,12,13,14-octahydro-10,10,11,12,12-D5-6-oxa-benzocyclotetradecen-5-one; (-)-α-Zearalenol-d5; Zearalenol-d5; trans-Zearalenol-d5; (3S,7R,11E)-3,4,5,6,7,8,9,10-Octahydro-7,14,16-trihydroxy-3-methyl-1H-2-benzoxacyclotetradecin-1-one-d5 |
| Storage | Store at -20°C under inert atmosphere |
| IUPAC Name | (4S,8R,12E)-7,7,8,9,9-pentadeuterio-8,16,18-trihydroxy-4-methyl-3-oxabicyclo[12.4.0]octadeca-1(14),12,15,17-tetraen-2-one |
| Canonical SMILES | CC1CCCC(CCCC=CC2=C(C(=CC(=C2)O)O)C(=O)O1)O |
| InChI | InChI=1S/C18H24O5/c1-12-6-5-9-14(19)8-4-2-3-7-13-10-15(20)11-16(21)17(13)18(22)23-12/h3,7,10-12,14,19-21H,2,4-6,8-9H2,1H3/b7-3+/t12-,14+/m0/s1 |
| InChI Key | FPQFYIAXQDXNOR-QDKLYSGJSA-N |
| Source | Fusarium sp. |
Properties
| Appearance | Light Beige Solid |
| Boiling Point | 599.0±50.0°C (Predicted) |
| Melting Point | >144°C (dec.) |
| Density | 1.174±0.06 g/cm3 (Predicted) |
| Solubility | Soluble in Acetone (Slightly), Methanol (Slightly) |
Toxicity
| Carcinogenicity | No indication of carcinogenicity to humans (not listed by IARC). |
| Mechanism Of Toxicity | Mycotoxins, such as α-zearalenol (α-ZOL) and β-Zearalenol (β-ZOL), as contaminants of animal food can impair fertility and can cause abnormal fetal development in farm animals. The effects of mycotoxins on FSH receptor-dependent and receptor-independent pathways indicate that adenylate cyclase activity and/or regulatory pathways further downstream are targets of mycotoxin actions. The apparent dose-dependent reduction of p450scc and 3beta-HSD transcripts implies an effect of α- and β-ZOL on transcriptional regulation of these enzymes. The zearalenone metabolites induced loss of mitochondrial membrane potential (MMP), mitochondrial changes in Bcl-2 and Bax proteins, and cytoplasmic release of cytochrome c and apoptosis-inducing factor (AIF). Use of an inhibitor specific to c-Jun N-terminal kinase (JNK), p38 kinase or p53, but not pan-caspase or caspase-8, decreased the toxin-induced generation of reactive oxygen species (ROS) and also attenuated the α-ZOL- or β-ZOL-induced decrease of cell viability. |
Reference Reading
1. Zearalenone (ZEN) in Livestock and Poultry: Dose, Toxicokinetics, Toxicity and Estrogenicity
Jundi Liu, Todd Applegate Toxins (Basel) . 2020 Jun 7;12(6):377. doi: 10.3390/toxins12060377.
One of the concerns when using grain ingredients in feed formulation for livestock and poultry diets is mycotoxin contamination. Aflatoxin, fumonisin, ochratoxin, trichothecene (deoxynivalenol, T-2 and HT-2) and zearalenone (ZEN) are mycotoxins that have been frequently reported in animal feed. ZEN, which has raised additional concern due to its estrogenic response in animals, is mainly produced byFusariumgraminearum(F.graminearum),F.culmorum,F.cerealis,F.equiseti,F.crookwellenseandF.semitectums, and often co-occurs with deoxynivalenol in grains. The commonly elaborated derivatives of ZEN are -zearalenol, -zearalenol, zearalanone, -zearalanol, and -zearalanol. Other modified and masked forms of ZEN (including the extractable conjugated and non-extractable bound derivatives of ZEN) have also been quantified. In this review, common dose of ZEN in animal feed was summarized. The absorption rate, distribution ("carry-over"), major metabolites, toxicity and estrogenicity of ZEN related to poultry, swine and ruminants are discussed.
2. Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach
Lorella Severino, Stefania Marzocco, Susan Costantini, Francesca Capone, Angela Sorice, Anna Chiara Nittoli, Roberto Ciarcia, Alfredo Budillon Metabolomics . 2018 Feb 14;14(3):33. doi: 10.1007/s11306-018-1330-3.
Introduction:Zearalenone (ZEN) is one of the most widely distributed toxins that contaminates many crops and foods. Its major metabolites are α-Zearalenol (α-zol) and β-Zearalenol. Previous studies showed that ZEN and α-zol have estrogenic properties and are able to induce growth promoting effect in breast tissues.Objectivies:Considering that tumorigenesis is dependent on the reprogramming of cellular metabolism and that the evaluation of the cellular metabolome is useful to understand the metabolic changes that can occur during the cancer development and progression or after treatments, aim of our work is to study, for the first time, the effects of α-zol on the metabolomic profile of an estrogen positive breast cancer cell line, MCF-7, and of an estrogen negative breast cancer cell lines MDA-MB231.Methods:Firstly, we tested the effects of α-zol on the cell viability after 24, 48 and 72 h of treatments with 10-10, 10-8and 10-6M concentrations on breast cancer MCF-7 and MDA-MB231 cell lines in comparison to human non-cancerous breast MCF10A cell line. Then, we evaluated cell cycle progression, levels of reactive oxygen species (ROS) and the metabolomic profiling by1H-NMR approach on MCF-7 and MDA-MB231 before and after 72 h treatments. Principal component analysis was used to compare the obtained spectra.Results:α-zol is resulted able to induce: (i) an increase of the cell viability on MCF-7 cells mainly after 72 h treatment, (ii) a slight decrease of the cell viability on MDA-MB231 cells, and (iii) an increase of cells in S phase of the cell cycle and of ROS only in MCF-7 cells. Moreover, the evaluation of metabolomics profile evidenced that after treatment with α-zol the levels of some metabolites increased in MCF-7 cells whereas decreased slightly in MDA-MB231 cells.Conclusions:Our results showed that α-zol was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression.
3. Toxicokinetics of α-zearalenol and its masked form in rats and the comparative biotransformation in liver microsomes from different livestock and humans
Marthe De Boevre, Feifei Sun, Sarah De Saeger, Jinhui Zhou, Shupeng Yang, Yanshen Li, Huiyan Zhang, Yi Li J Hazard Mater . 2020 Jul 5;393:121403. doi: 10.1016/j.jhazmat.2019.121403.
Alpha-zearalenol (α-ZEL) and its masked form α-zearalenol-14 glucoside (α-ZEL-14G) have much higher oestrogenic activity than zearalenone. Owing to very limited toxicokinetic and metabolic data, no reference points could be established for risk assessment. To circumvent it, the toxicokinetic, metabolic profiles, and phenotyping of α-ZEL and α-ZEL-14G were comprehensively investigated in this study. As a result, the plasma concentrations of α-ZEL and α-ZEL-14G were all below LOQ after oral administration, while after iv injection, both could be significantly bio-transformed into various metabolites. A complete hydrolysis of α-ZEL-14G contributed to α-ZEL overall toxicity. Additionally, 31 phase I and 10 phase II metabolites of α-ZEL, and 9 phase I and 5 phase II metabolites were identified for α-ZEL-14G. For α-ZEL, hydroxylation, dehydrogenation, and glucuronidation were the major metabolic pathways, while for α-ZEL-14G, it was deglycosylation, reduction, hydroxylation, and glucuronidation. Significant metabolic differences were observed for α-ZEL and α-ZEL-14G in the liver microsomes of rats, chickens, swine, goats, cows and humans. Phenotyping studies indicated that α-ZEL and α-ZEL-14G were mediated by CYP 3A4, 2C8, and 1A2. Moreover, the deglycosylation of α-ZEL-14G was critically mediated by CES-I and CES-II. The acquired data would provide fundamental perspectives for risk evaluation of mycotoxins and their modified forms.
Spectrum
Predicted LC-MS/MS Spectrum - 10V, Positive
Experimental Conditions
Ionization Mode: Positive
Collision Energy: 10 eV
Instrument Type: QTOF (generic), spectrum predicted by CFM-ID
Mass Resolution: 0.0001 Da
Collision Energy: 10 eV
Instrument Type: QTOF (generic), spectrum predicted by CFM-ID
Mass Resolution: 0.0001 Da
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