Herboxidiene
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| Category | Others |
| Catalog number | BBF-01717 |
| CAS | 142861-00-5 |
| Molecular Weight | 438.60 |
| Molecular Formula | C25H42O6 |
| Purity | 98% |
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Description
Herboxidiene is a substance with herbicidal activity produced by Streptomyces chromofuscus. It has selective and powerful weeding effect on a variety of monocot and dicot weeds.
Specification
| Synonyms | Herboxidiene |
| Storage | RT |
| IUPAC Name | 2-[(2R,5S,6S)-6-[(2E,4E,6S)-7-[(2R,3R)-3-[(2R,3R,4R)-4-hydroxy-3-methoxypentan-2-yl]-2-methyloxiran-2-yl]-6-methylhepta-2,4-dien-2-yl]-5-methyloxan-2-yl]acetic acid |
| Canonical SMILES | CC1CCC(OC1C(=CC=CC(C)CC2(C(O2)C(C)C(C(C)O)OC)C)C)CC(=O)O |
| InChI | InChI=1S/C25H42O6/c1-15(14-25(6)24(31-25)18(4)23(29-7)19(5)26)9-8-10-16(2)22-17(3)11-12-20(30-22)13-21(27)28/h8-10,15,17-20,22-24,26H,11-14H2,1-7H3,(H,27,28)/b9-8+,16-10+/t15-,17+,18-,19-,20-,22-,23-,24-,25-/m1/s1 |
| InChI Key | ISZXEMUWHQLLTC-LSIVYLFASA-N |
Properties
| Appearance | Off-White Solid |
| Boiling Point | 567.9°C at 760 mmHg |
| Density | 1.056 g/cm3 |
| Solubility | Soluble in Methanol, Water |
Reference Reading
1. Herboxidiene triggers splicing repression and abiotic stress responses in plants
Sahar AlShareef, Yu Ling, Haroon Butt, Kiruthiga G Mariappan, Moussa Benhamed, Magdy M Mahfouz BMC Genomics. 2017 Mar 27;18(1):260. doi: 10.1186/s12864-017-3656-z.
Background: Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results: Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions: Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.
2. Herboxidiene Features That Mediate Conformation-Dependent SF3B1 Interactions to Inhibit Splicing
Adriana Gamboa Lopez, Srinivasa Rao Allu, Patricia Mendez, Guddeti Chandrashekar Reddy, Hannah M Maul-Newby, Arun K Ghosh, Melissa S Jurica ACS Chem Biol. 2021 Mar 19;16(3):520-528. doi: 10.1021/acschembio.0c00965. Epub 2021 Feb 22.
Small molecules that target the spliceosome SF3B complex are potent inhibitors of cancer cell growth. The compounds affect an early stage of spliceosome assembly when U2 snRNP first engages the branch point sequence of an intron. Employing an inactive herboxidiene analog (iHB) as a competitor, we investigated factors that influence inhibitor interactions with SF3B to interfere with pre-mRNA splicing in vitro. Order-of-addition experiments show that inhibitor interactions are long lasting and affected by both temperature and the presence of ATP. Our data are also consistent with the model that not all SF3B conformations observed in structural studies are conducive to productive inhibitor interactions. Notably, SF3B inhibitors do not impact an ATP-dependent rearrangement in U2 snRNP that exposes the branch binding sequence for base pairing. We also report extended structure-activity relationship analysis of the splicing inhibitor herboxidiene. We identified features of the tetrahydropyran ring that mediate its interactions with SF3B and its ability to interfere with splicing. In the context of recent structures of SF3B bound to inhibitor, our results lead us to extend the model for early spliceosome assembly and inhibitor mechanism. We postulate that interactions between a carboxylic acid substituent of herboxidiene and positively charged SF3B1 side chains in the inhibitor binding channel are needed to maintain inhibitor occupancy while counteracting the SF3B transition to a closed state that is required for stable U2 snRNP interactions with the intron.
3. Design and synthesis of herboxidiene derivatives that potently inhibit in vitro splicing
Arun K Ghosh, Srinivasa Rao Allu, Guddeti Chandrashekar Reddy, Adriana Gamboa Lopez, Patricia Mendez, Melissa S Jurica Org Biomol Chem. 2021 Feb 18;19(6):1365-1377. doi: 10.1039/d0ob02532a.
Herboxidiene is a potent antitumor agent that targets the SF3B subunit of the spliceosome. Herboxidiene possesses a complex structural architecture with nine stereocenters and design of potent less complex structures would be of interest as a drug lead as well as a tool for studying SF3B1 function in splicing. We investigated a number of C-6 modified herboxidiene derivatives in an effort to eliminate this stereocenter and, also to understand the importance of this functionality. The syntheses of structural variants involved a Suzuki-Miyaura cross-coupling reaction as the key step. The functionalized tetrahydrofuran core has been constructed from commercially available optically active tri-O-acetyl-d-glucal. We investigated the effect of these derivatives on splicing chemistry. The C-6 alkene derivative showed very potent splicing inhibitory activity similar to herboxidiene. Furthermore, the C-6 gem-dimethyl derivative also exhibited very potent in vitro splicing inhibitory activity comparable to herboxidiene.
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Bio Calculators
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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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Tip: Chemical formula is case sensitive. C22H30N4O √ c22h30n40 ╳
