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Hygromycin A

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Hygromycin A
Category Others
Catalog number BBF-00969
CAS 6379-56-2
Molecular Weight 511.48
Molecular Formula C23H29NO12
Purity 95%
Catalog Number Size Price Stock Quantity
BBF-00969 25 mg $399 In stock

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Fermentation Lab

4 R&D and scale-up labs

2 Preparative purification labs

Fermentation Plant

Semi pilot, pilot and industrial plant 4 Manufacturing sites 7 Production lines at pilot scale 100+ Reactors of 30-4000 L; 170+ reactors of 20 KL-30 KL; 24+ reactors of >100 KL 2 Hydrogenation reactors (200 L, 4Mpa and 1000L, 4Mpa)

Product Description

Hygromycin A is a natural antibiotic found in Streptomyces species. Hygromycin A selectively kills the bacteria that cause Lyme disease.

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Synonyms Totomycin; Antibiotic WS-1627B; 4-(5-Acetyl-3,4-dihydroxy-2-tetrahydrofuranyloxy)-3-hydroxy-alpha-methyl-N-(2,3,6-trihydroxy-4,5-methylendioxycyclohexyl)zimtsaeureamid
Storage Store at -20 °C
IUPAC Name (E)-N-[(3aS,4R,5R,6S,7R,7aR)-4,6,7-trihydroxy-3a,4,5,6,7,7a-hexahydro-1,3-benzodioxol-5-yl]-3-[4-[(2S,3S,4S,5S)-5-acetyl-3,4-dihydroxyoxolan-2-yl]oxy-3-hydroxyphenyl]-2-methylprop-2-enamide
Canonical SMILES C/C(=C\C1=CC(=C(C=C1)O[C@H]2[C@H]([C@@H]([C@H](O2)C(=O)C)O)O)O)/C(=O)N[C@@H]3[C@@H]([C@H]([C@@H]4[C@H]([C@@H]3O)OCO4)O)O
InChI InChI=1S/C23H29NO12/c1-8(22(32)24-13-14(27)16(29)21-20(15(13)28)33-7-34-21)5-10-3-4-12(11(26)6-10)35-23-18(31)17(30)19(36-23)9(2)25/h3-6,13-21,23,26-31H,7H2,1-2H3,(H,24,32)/b8-5+/t13-,14+,15-,16-,17+,18+,19-,20+,21-,23-/m1/s1
InChI Key YQYJSBFKSSDGFO-IIHALWDASA-N
Appearance White to Light Yellow Solid
Antibiotic Activity Spectrum Gram-positive bacteria; mycobacteria; mycoplasma; parasites
Boiling Point 925.5±65.0 °C (Predicted)
Melting Point 105-109 °C
Density 1.60±0.1 g/cm3 (Predicted)
Solubility Soluble in DMF, DMSO, Ethanol
1. [Effect of hepatocyte growth factor gene transfection on biological features of lymphoma cells]
Dong Cen, Hang Zhao, Rong-rong Shen, Shi-xuan Hua, Jian-xin Lu, Ren-zhi Pei, Zhi-guang Tu Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Jul;39(4):378-85. doi: 10.3785/j.issn.1008-9292.2010.04.007.
Objective: To investigate the biological effect of hepatocyte growth factor (HGF) on HGF gene-transfected Raji cells. Methods: Total RNA was extracted from human hepatic tissue, HGF gene cDNA was amplified by RT-PCR, and then cloned into vector pVITRO2-mcs to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF. The recombinant vector was transfected to Raji cells, and the stably transfected cells were selected by homomycin B in serial passages, and confirmed by real-time fluorescent quantitative PCR, ELISA, immunocytohistochemistry. The biological features of transfected Raji cells were evaluated by semisolid culture. Results: RT-PCR results showed that Raji cells were transfected successfully with recombinant eukaryotic expression vector pVITRO2-mcs-HGF. HGF mRNA and protein were expressed successfully in Raji cells. Expression of HGF gene enhanced proliferation, metastasis and invasion of Raji cells. Conclusion: HGF gene has been cloned and recombined to construct recombinant eukaryotic expression vector pVITRO2-mcs-HGF successfully. Transfected HGF may change the biological features of Raji cells.

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