Leptolstatin
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| Category | Enzyme inhibitors |
| Catalog number | BBF-02249 |
| CAS | 149633-91-0 |
| Molecular Weight | 498.69 |
| Molecular Formula | C31H46O5 |
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Description
It is produced by the strain of Str. sp. SAM1595. It has no activity against bacteria, fungi and yeast, in addition to Millet wine schizozoite yeast (MIC is 0.4 μg/mL). It has a strong inhibitory effect on mammalian cells, such as normal fibroblast 3Y1 cells in rats (IC50 is 0.4 ng/mL).
Specification
| Synonyms | 2H-Pyran-2-one, 6-[(1E,3Z,5R,7E,9E,11R,13S,14R,15S,17E)-14,19-dihydroxy-3,5,9,11,13,15,17-heptamethyl-12-oxo-1,3,7,9,17-nonadecapentaen-1-yl]-5,6-dihydro-, (6R)-; (R)-6-((1E,3Z,5R,7E,9E,11R,13S,14R,15S,17E)-14,19-dihydroxy-3,5,9,11,13,15,17-heptamethyl-12-oxononadeca-1,3,7,9,17-pentaen-1-yl)-5,6-dihydro-2H-pyran-2-one |
| IUPAC Name | (2R)-2-[(1E,3Z,5R,7E,9E,11R,13S,14R,15S,17E)-14,19-dihydroxy-3,5,9,11,13,15,17-heptamethyl-12-oxononadeca-1,3,7,9,17-pentaenyl]-2,3-dihydropyran-6-one |
| Canonical SMILES | CC(CC=CC(=CC(C)C(=O)C(C)C(C(C)CC(=CCO)C)O)C)C=C(C)C=CC1CC=CC(=O)O1 |
| InChI | InChI=1S/C31H46O5/c1-21(18-23(3)14-15-28-12-9-13-29(33)36-28)10-8-11-22(2)19-25(5)30(34)27(7)31(35)26(6)20-24(4)16-17-32/h8-9,11,13-16,18-19,21,25-28,31-32,35H,10,12,17,20H2,1-7H3/b11-8+,15-14+,22-19+,23-18-,24-16+/t21-,25-,26+,27-,28-,31-/m1/s1 |
| InChI Key | LMVRPBWWHMVLPC-OATZMCNESA-N |
Properties
| Appearance | Colorless Oily Matter |
| Antibiotic Activity Spectrum | Yeast |
| Solubility | Soluble in Methanol |
Reference Reading
1. Leptolstatin from Streptomyces sp. SAM1595, a new gap phase-specific inhibitor of the mammalian cell cycle. II. Physico-chemical properties and structure
K Abe, M Yoshida, H Naoki, S Horinouchi, T Beppu J Antibiot (Tokyo). 1993 May;46(5):735-40. doi: 10.7164/antibiotics.46.735.
Leptolstatin, a product from Streptomyces sp. SAM1595, is a gap phase-specific inhibitor of the mammalian cell cycle. Physico-chemical properties and spectrometric analyses showed that the structure of leptolstatin is (2Z,6E,8Z,12E,14E,22E)-19,24-dihydro xy-8,10,14,16,18,20,22-heptamethyl-17-oxo-2 , 6,8,12,14,22-tetracosahexaen-5-olide.
2. Leptolstatin from Streptomyces sp. SAM1595, a new gap phase-specific inhibitor of the mammalian cell cycle. I. Screening, taxonomy, purification and biological activities
K Abe, M Yoshida, S Horinouchi, T Beppu J Antibiot (Tokyo). 1993 May;46(5):728-34. doi: 10.7164/antibiotics.46.728.
Leptolstatin, a new inhibitor of the progression of G1 and G2 phases of the mammalian cell cycle, was discovered through a unique screening system, in which effects of microbial metabolites on the cell cycle progression of the cultured rat fibroblasts were monitored by flow cytometry. The new inhibitor was extracted from the fermentation broth of Streptomyces sp. SAM1595 with ethyl acetate, and purified by silica gel column chromatography and HPLC. Leptolstatin showed a strong cytostatic effect on rat normal fibroblasts 3Y1 with an IC50 value of 0.4 ng/ml, but its antimicrobial activity was very weak. A 24-hour treatment of the fibroblast cells with 10 ng/ml of leptolstatin caused an arrest at G1 or G2 phase, as determined by flow cytometry. When the G2-arrested cells were freed from leptolstatin, those containing 4C DNA entered a new S phase without intervening M phase, resulting in the formation of proliferative tetraploid cells.
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Bio Calculators
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
* Total Molecular Weight:
g/mol
Tip: Chemical formula is case sensitive. C22H30N4O √ c22h30n40 ╳
