Strobilurin F

Strobilurin D and strobilurin F, two new cytostatic and antifungal antibiotics of the (E)-beta-methoxyacrylate class, have been isolated from mycelial cultures of the basidiomycete Cyphellopsis anomala (Pers. ex Fr.) Sing. The structures were elucidated by spectroscopic methods. The antibiotics inhibit the growth of human and animal cells and a wide variety of fungi. Like strobilurins A and B, the new metabolites are potent inhibitors of respiration.

Three new strobilurins F, G and H, antibiotics with antifungal activity, were isolated from cultures of Bolinea lutea Sacc. These new compounds differ from previously described analogs in their aromatic substitution. An HPLC method allows complete separation of all the components.

A mycelial co-metabolite of strobilurin A, F and G from Bolinea lutea, which we have named bolineol, has been characterised for the first time. Bolineol (methyl 2-hydroxymethyl-3-methyl-6-phenyl-3Z,5E-hexadienoate) has a similar carbon skeleton to strobilurin A but differs in oxidation level. Its structure has been deduced from its spectral characteristics and those of its acetate. The known compound strobilurin F-1 (methyl 2-methoxymethylene-3-methyl-6-(3'-hydroxyphenyl)-3Z,5E-hexadienoate) has been detected in this organism for the first time and converted into its acetate, a new compound, the structure of which was assigned from its spectral data.