Fermentation CDMO Service for Pharmaceutical Industry

Within the pharmaceutical field, fermentation refers to culturing microbial strains (usually fungi, yeast and E. coil) to overproduce crucial compounds, such as therapeutic proteins, antibodies, antibiotics, active pharmaceutical ingredients (APIs) and other valuable small molecules.

BOC Sciences provides comprehensive microbial fermentation services with our advanced fermentation technology and comprehensive strain development platform. The ultimate mass manufacturing and production goal for effective chemical and biochemical can be achieved to meet customer needs.

Our Methods

BOC Sciences offers fermentation CDMO services to our global partners from influential pharma, biotech, and biopharma. We are capable of providing one-stop microbial fermentation services from concept to commercialization.

Strain development/improvement service

Strain development or strain improvement is a technology that improves microbial strains to enhance metabolic capacities or obtain desirable microbes. Improved microbial strains solve specific obstacles by manipulating and modifying the genome, including mutagenesis, gene cloning, genetic recombination, gene editing, transfection and other molecular biology methods.

Upstream and downstream fermentation process optimization

Our upstream fermentation process optimization includes strain improvement, medium selection, strains culture condition selection, etc. Our downstream fermentation process optimization includes fermentation processing scale-up, separation and purification with products of interest, protein recovery, etc.

Strain screening and compound characterization

Our strains expression systems cover bacterial systems (E. coli, Streptomyces, Bacillus subtilis) and eukaryotic systems (Aspergillus, Pichia pastoris, Saccharomyces cerevisiae, etc.). We are capable of screening ideal strains and characterizing metabolites produced by strains with our leading analytical capabilities.

GMP complied fermentation production

Our quality control extends through every step of the fermentation process, from laboratory to production facilities are GMP compliant. Furthermore, BOC Sciences provides microbial GMP production services.

Fermentation in Pharmaceutical Industry

Fermentation can be used to produce pharmaceutical products, including but not limited to:

• Active Pharmaceutical Ingredients (APIs)
• Antibodies
• Enzymes
• Plasmid DNA
• Therapeutic proteins
• Viral vector
• Other unique small molecules, such as short-chain peptides, steroids, opioids, etc.

Fermentation for Biologicals

Microorganisms commonly used in the pharmaceutical industry include prokaryotes, such as E. coli, to produce pharmaceutical products efficiently and economically. Among eukaryotes, Pichia pastoris combines the growth characteristics of prokaryotes with the protein post-translational modification (PTM) characteristics of eukaryotic cells, and such microbial fermentation has been widely used in vaccines, antibody fragments, hormones, cytokines, recombinant proteins and biosimilars. Nowadays, increasing antibodies or protein drugs are produced by microbial cells. For example, E. coli is one of the most frequently used microbes in recombinant proteins production. BOC Sciences revealed extensive experiences in protein expressions, such as the expression of plasmids, strains and cultivation strategies.

Fermentation for Gene Therapy

Plasmid-based gene therapy requires massive purified plasmid production, and E. coli is generally served as the organism for fermentative production of plasmid DNA. Moreover, in gene therapy, the production of viral vectors is often the most expensive supply chain component. Thus, transient transfection of plasmid DNA into a host cell line for vector production can significantly reduce costs. BOC Sciences supports plasmid DNA and viral vectors production by culturing microbial strains and mammalian cells.

Workflow of CDMO Services

BOC Sciences provides one-stop fermentation development services from concept to commercialization.

• Project design and evaluation
• Strain selection and development
• Media and process optimization
• Fermentation and scale-up
• Project delivery