Fermentation for Antiparasitic Agents
Recently, parasite constitutes have posed a serious problem and become a major threat to animal production, health and welfare. Parasite infections can seriously affect livestock and these infections have a severe impact on the immune system, which in turn can lead to serious economic losses and animal deaths. In recent decades, the control of parasitic diseases has been achieved mainly through the use of anti-parasitic agents.
Hazards of Parasites
- Reduced voluntary intake
- Reduced productivity indices including live weight gain, milk production, wool production, etc.
- Reduced product quality due to high protein losses
Fermentation Techniques in Antiparasitic Agents Production
Standard fermentation and isolation techniques are applied to isolate paraherquamide and six novel analogues from the fermentation process of Penicillium charlesii. All of the obtained products have showed high activity against Caenorhabditis elegans.
In animal studies, megalomicin was shown to have extremely low toxicity, raising the prospect that derivatives could also be developed into potent drugs for the treatment of malarial disease. As a well-characterized host, Saccharopolyspora erythrae is a well-established genetic engineering tool that can be applied to a wide range of fermentation developments. Thus, the conversion of Saccharopolyspora erythrae into a meglomicin producer makes it possible to generate new antiparasitic compound by employing the fermentation engineering. The erythromycin (ery) genes are employed as probes to target the megalomicin (meg) biosynthetic clusters, and these meg clusters contain genes for the synthesis of the unique deoxysugar l-megosamine. Finally, the expression of these genes in Saccharopolyspora erythrae will lead to the production of megalomicin.
Fig 1. Potential antiparasitic targets. (Kayamba, F.; et al. 2021)
Isolation of microorganisms from various soil samples, fermentation of such microorganisms under appropriate conditions as well as screening of the resulting fermentation broths or partially purified extracts is a long-standing popular fermentation method for finding antiparasitic agents with novel chemical structures that show desirable biological activity. The spinosyns are compounds produced via the fermentation of two species of Saccharopolyspora. Their core structure is a tetracyclic macrolide of polyketide derivatives with two saccharides attached. It has been shown that the spinosyns show effective insecticidal activity against a variety of species that cause extensive damage to crops and other plants. They have also shown activity against important external parasites of livestock and companion animals.
Moreover, a novel dinaphthyl compound has been successfully produced via the fermentation process. This antiparasitic compound is found to have antiparasitic and insecticidal activity. The whole aerobic fermentation process is carried out using Xanthospora aureus in a suitable aqueous nutrient medium containing trace metals necessary for microbial growth and production of the desired compounds, these are usually present in sufficient concentrations in complex carbon and nitrogen sources which can be used as nutrient sources. Finally, this antiparasitic compound is separated and recovered from the whole fermentation broth by solvent extraction and application of chromatographic fractionation with various chromatographic techniques and solvent systems.
Our Services
- Fermentation CDMO Service
- Strain Development Service
- Fermentation Process Optimization
- Fermentation for Special Small Molecules
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BOC Sciences provides fermentation CDMO service for antiparasitic agents. With our complete quality management system and strong fermentation capabilities, we are able to help customers facilitate their fermentation related projects and support them from laboratory to commercialization.
Workflow of Our Service
Reference
- Kayamba, F.; et al. Lactate dehydrogenase and malate dehydrogenase: Potential antiparasitic targets for drug development studies. Bioorganic & Medicinal Chemistry. 2021. 20(15): 116458.
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