Fermentation for Living Probiotics

Special types of bacteria associated with "healthy" fermentation in the human gut are classified as "probiotics". Probiotics are products containing sufficient quantities of live microorganisms (mainly lactobacilli and bifidobacteria) to alter the microbiota in the host and confer beneficial health effects, which are often strain-specific. Typically, probiotics are used in therapeutic preparations and added to food. In addition to the stimulating effect on the immune system, the benefits of regular consumption of probiotics include reduced risk of colon cancer, cholesterol levels, incidence of diarrhea, etc. In addition to being functional ingredients, probiotics are also sold as health supplements in the form of pills and liquid formulations.

BOC Sciences is one of the world leaders in probiotics. Our core business is to develop, produce and promote high-quality probiotic raw materials, functional ingredients and excipients, including vitamins, minerals, flavors, excipients, etc., to provide practical solutions for the use of probiotics.

Probiotic Fermentation Technologies

Continuous fermentation

Continuous culture can result in high cell yields and process volume productivity, as well as reduced requirements for downstream processing capacity. Under steady-state operation, cells produced in continuous culture are in a controlled physiological state that can be manipulated by environmental parameters such as media composition and dilution rate, which determine the specific growth rate of microorganisms in the system.

Membrane bioreactor

In membrane systems with continuous input of fresh medium, cells are retained in the bioreactor by ultrafiltration or microfiltration membranes, while small molecules diffuse through the pores of the membranes according to their size. Thus, inhibitory metabolites are eliminated in the permeate and cells are concentrated in the reflux. Concentrated cell fractions can be harvested batchwise or continuously, requiring no or minimal downstream processing to concentrate cells prior to freezing or lyophilization. These systems can generate high cell yields and volumetric productivity.

Our Services of Fermentation for Probiotics

As the main manufacturer and supplier of probiotics and their derivatives in the world, we have a number of probiotic factories and have established cooperative relationships with several research centers, so we can provide customers in different countries with the best quality services.

Strain selection: Strain selection is the first important step in the probiotic production process. We supply high-quality probiotic raw materials. Genera of commonly used probiotics include lactic acid bacteria, bifidobacteria, bacillus, streptococcus, etc.

Media Formulation: We help develop effective recipes and combinations of process parameters for the correct dosage of raw materials and test them for gut viability. After optimization, the selected strains were fermented and stabilized.

Fermentation: The strains are fermented in fermenters that contain a nutrient-rich and warm bath of ingredients. During fermentation, all nutrients and equipment are sterilized to eliminate any unnecessary and accidental contamination.

Separation and Purification: Metabolites are formed during fermentation, which are by-products of bacteria metabolizing nutrients. We separate probiotic strains and metabolites through separation and purification technology. Freeze-drying or spray-drying is used to maintain the stability and potency of supplements for long-term storage.

Probiotics analysis technology and equipment

• Flow cytometry and fluorescence in situ hybridization
• DNA microarray
• Transcriptomics, Proteomics and Metabolomics
• PCR method

What are the function of probiotics?

• Promote digestion and absorption.
• Strengthen immune cells and protect intestinal mucosa.
• Reduce the risk of cancer.
• Reduce cholesterol absorption.
• Auxiliary anti-oxidation of oxides in the body.
• Moisten the intestines and laxative.

We use our rich experience and scientific verification to provide customers with probiotic fermentation and related chemical ingredients, and drive the long-term development of our partners through the best service quality.

If you are interested in our fermentation services for living probiotics, please contact us today.

References

1. Rodgers S. Novel applications of live bacteria in food services: probiotics and protective cultures. Trends in Food Science & Technology. 2008 Apr 1;19(4):188-97.
2. Lacroix C, Yildirim S. Fermentation technologies for the production of probiotics with high viability and functionality. Current Opinion in Biotechnology. 2007 Apr 1;18(2):176-83.