Pseudomonas fluorescens Fermentation Services

BOC Sciences offers comprehensive microbial fermentation services, which range from lab fermentation to industrial manufacturing. Based on advanced strain improvement technology and extensive fermentation experience, we provide one-stop fermentation services to support R&D and help accelerate the commercialization of our customers' products. Pseudomonas fluorescens, an important engineered host strain improved by extensive molecular biology and bioinformatics tools, can manufacture high yield non-glycosylated protein. In addition, we also utilize Pseudomonas fluorescens as a host strain to ferment and produce valuable secondary metabolites.

Examples of Pseudomonas fluorescens fermentation products:

Introduction of P. fluorescens

Pseudomonas fluorescens, abbreviated as P. fluorescens, is a common Gram-negative bacteria usually found in moist environments such as soil, leaves, and water. Typically, P. fluorescens is non-pathogenic, so it does not cause disease in humans. P. fluorescens is a facultative anaerobe capable of using inorganic compounds such as nitrate and nitrite instead of oxygen as alternate terminal electron receptors. P. fluorescens is a chemoorganotrophic that grows between pH 4 and 8. P. fluorescens is a psychrophile microorganism that grows at an optimal temperature of 25-30℃. P. fluorescens is a rod-shaped bacteria with cells arranged singly and not linked in chains. It is also a motile bacteria and thus, retains the presence of flagella.

As its name implies, P. fluorescens secretes a soluble green fluorescent pigment called pyoverdin, especially under conditions of low iron availability. Fluorescent bacteria are potential biocontrol agents that suppress plant diseases by protecting roots and seeds from fungal infections. They are known to promote plant growth and reduce the severity of various fungal diseases. In addition, P. fluorescens is a powerful and effective active recombinant protein that can operate as a production system, and some protein drugs produced by P. fluorescens have entered clinical applications.

Advantages of P. fluorescens Fermentation

Growth of P. fluorescens

P. fluorescens has an extremely broad metabolic capacity, giving this bacteria the ability to persist in various environments. P. fluorescens can grow on simple, inexpensive media. It has simple nutritional requirements and grows well in mineral salts media and as supplemented with a large carbon source.

Production of P. fluorescens

One of the main mechanisms by which P. fluorescens suppresses plant pathogens is through the production of various secondary metabolites capable that are able to inhibiting fungal growth. Importantly, P. fluorescens fermentation produces a specific antibiotic called mupirocin that effectively treats certain diseases.

Safety of P. fluorescens

P. fluorescens is a biosafety level 1 organism that has been used for years to produce proteins for therapeutic applications. It is worth mentioning that several proteins produced by P. fluorescens have entered clinical trials in the United States and other countries.

Applications of P. fluorescens Fermentation

P. fluorescens is a powerful host of protein expression and can express secreted proteins at high yields, which can also direct the secretion of high protein levels into the periplasm. This process allows for increased efficiency and more diverse mechanisms of protein oxidation, folding, and quality control. Within correctly folded expressions, active targets can eliminate the need for complex and expensive refolding steps. If the expressed protein does not require glycosylation, bacterial expression systems are preferred and can offer advantages in both speed and cost. As a result, P. fluorescens is a reliable recombinant protein expression system for the biopharmaceutical industry.

P. fluorescens belongs to the plant growth-promoting rhizobacteria (PGP) group and plays an important role in promoting plant growth, inducing systemic resistance, and biocontrol of pathogens. Some P. fluorescens are potential biocontrol agents that suppress plant diseases through their protection of seeds and roots from fungal infections. P. fluorescens fermentation produces a number of secondary metabolites for agricultural application, ranging from antibiotics, siderophores, and hydrogen cyanide, in order to promote plant growth and reduce the severity of many fungal diseases.

Project Workflow of P. fluorescens Fermentation

Customer advisory
Project discussion
P. fluorescens served as host
Strain improvement and fermentation development
Novel strain evaluation
Project delivery

References

Ganeshan, G. and Kumar, A. M., Pseudomonas fluorescens, a potential bacterial antagonist to control plant diseases, J. Plant Interact., 2005, 1, 123-134.
Mishra, J., Arora, N. K., Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture, Appl. Soil Ecol., 2018, 125, 35-45.
Retallack, D. M., Jin, H., Chew, L., Reliable protein production in a Pseudomonas fluorescens expression system, Protein Expr. Purif., 2012, 81, 157-165.