Metabolites-BOC Sciences

Strain Development Service

Strain Development Service

BOC Sciences offers strain development and strain improvement services to enhance their metabolic capacities. With over 20 years of experience in traditional strain technology, we are capable of providing our customers with ideal strains whose advantages include rapid growth, genetic stability, and reduction of cultivation costs. Our strain development and improvement services cover various microbial systems, including Escherichia coli, streptomyces, actinomyces, mycobacterium and Paracoccus, and the eukaryotic systems such as yeast (Pichia pastoris, saccharomyces cerevisiae), aspergillus, penicillium, acremonium, filamentous fungi and excavate.

BOC Sciences has successfully developed and improved various strains for our customers and helped them transform primitive strains into industrialized strains effectively.

Introduction

In nature, microorganisms provide various valuable bioactive products, yet they produce a minimum amount of metabolites for survival. These metabolites are not overproduced naturally. However, overproduction of these metabolites revealed significant enhancement in the chemical, pharmaceutical, food industries. Therefore, it is essential to improve these microbial strains for industrial products.

The science and technology of manipulating and improving microbial strains by enhancing their metabolic capacities are known as strain development or strain improvement. Strain improvement is meant for obtaining microbial isolates possessing certain desirable characteristics for solving a specific problem. Traditionally, strain improvement has been achieved by mutation, selection, cloning and genetic recombination. Overproduction of primary or secondary metabolites is a complex process, and successful development of improved strains requires the combination of knowledge in physiology, molecular biology, genetic engineering, and the design of creative screening procedures.

Application of Strain Development

Microorganisms produce various essential compounds for the industrial, such as small molecules essential for vegetative growth (primary metabolites), and inessential (secondary metabolites), large molecules such as proteins, nucleic acids, carbohydrate polymers and more. Regulatory mechanisms have evolved in microorganisms that enable a strain to avoid an excessive production of metabolites; thus strain improvement programs are required for commercial application. The strain improvement strategy greatly increases fermentation productivity and significantly reduces costs. These genetic programs are also used for other goals, such as eliminating unwanted products or analogs, discovering novel structural compounds, and deciphering biosynthetic pathways.

Our Advantages

Over 20 years of experience in traditional strain breeding.

Provision of ideal strains characterized:

  • Rapid growth
  • Stabilized Genetic
  • Non-toxic to humans
  • Cost-effective substrates
  • Downstream processing interferences elimination
  • Improved carbon and nitrogen usages
  • Reduced cultivation cost
  • Shorten fermentation period

Advanced strain culture technology.

Optimized medium selection, biomass control and product induction.

Improved feedstock and feed regime.

Complete strain expression systems cover bacterial systems and eukaryotic systems.

Our Methods

Production strain choice and optimization

Developing an appropriate medium for fermentation

Mutation

  • Chemicals induced mutation
  • Radiation induced mutation
  • Site-directed mutagenesis

Genetic recombination is the exchange of genetic material between different organisms. There are three recombination techniques: transduction, transformation and conjugation

High-throughput screening and analytical technologies

  • Agar plate-based screening
  • Fluorescence-activated cell sorting
  • Droplet microfluidic screening

Project Workflow

  • Customer advisory
  • Project discussion
  • Selection of microbial host
  • Strain optimization: cloning, gene editing, mutation, screening, etc.
  • Evaluation of the novel strain, transform primitive strains into industrialized strains
  • Project delivery

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