Strain Improvement Services
We are a company specializing in fermentation CDMO services, dedicated to providing exceptional strain improvement services to our clients. We have an experienced professional team that utilizes advanced gene editing technologies and high-throughput screening platforms to precisely improve strains based on the specific needs of our clients. Whether it is enhancing the yield of target products, improving strain resistance to stress, or optimizing metabolic characteristics, we offer customized solutions to make fermentation processes more efficient and stable, helping our clients stand out in the competitive market and achieve significant production benefits.
Our Strain Improvement Services for fermentation
Strain Screening and Identification
Environmental Sample Strain Screening: We collect samples from various natural ecological environments such as soil, water, plants, and animal intestines, using selective media and high-throughput screening technology to isolate and select high-quality strains with specific fermentation or metabolic characteristics.
Strain Identification and Classification: Using methods such as 16S rRNA gene sequencing, whole-genome sequencing, and biochemical identification, we accurately identify and classify the selected strains, determining their position in the microbial classification system. This provides foundational information for subsequent strain improvement and application.
Strain Mutation Improvement
Physical Mutation: By using physical factors such as ultraviolet light, γ-rays, and electron beams, we treat strains to induce genetic mutations, thereby altering their metabolic pathways, fermentation product yield, and quality.
Chemical Mutation: We use chemical mutagens such as nitrites, diethyl sulfate, and hydroxylamine to treat strains, increasing the mutation frequency. We then combine these methods with screening to obtain mutant strains with superior fermentation performance. For example, nitrite treatment can be used to select yeast strains with stronger alcohol tolerance and faster fermentation speed for the brewing industry.
Genetically Engineered Strain Construction
Target Gene Cloning and Expression: Based on customer needs, we clone target genes with specific functions, such as genes encoding highly active enzymes or key synthesis enzymes for producing specific fermentation products, and introduce them into suitable host strains to create recombinant strains capable of efficiently expressing target proteins or synthesizing target fermentation products.
Metabolic Pathway Optimization: We use gene editing techniques to modify and optimize the metabolic pathways of strains, strengthening the synthesis pathway of target products, while weakening or blocking the formation of by-products. This improves the yield and quality of fermentation products.
Strain Adaptation Evolution
Environmental Stress Adaptation Evolution: We place strains under specific environmental stress conditions (such as high temperature, low temperature, high salt, high acid, high alkalinity, and oxygen deprivation) and conduct continuous multi-generation culturing, enabling the strains to gradually adapt to these harsh environments and enhancing their environmental tolerance and stability in actual fermentation production processes.
Substrate Adaptation Evolution: For specific fermentation substrates (such as lignocellulose, starch, molasses, and waste oils), we carry out adaptive evolution training to improve the strains' ability to utilize and convert these complex substrates, broadening their substrate spectrum and reducing fermentation costs.
Strain Preservation and Revitalization
Strain Preservation: We provide professional strain preservation services, using methods such as low-temperature preservation, freeze-drying, and liquid nitrogen storage. We choose the appropriate preservation method based on the strain's characteristics to ensure the survival rate and stability of strains during long-term storage.
Strain Revitalization: For strains that have been stored for extended periods or passaged multiple times, we carry out revitalization treatments to restore their excellent fermentation performance and biological properties, preventing degradation from negatively affecting fermentation production.
Strain Performance Evaluation and Optimization
Fermentation Performance Evaluation: We conduct fermentation trials under different conditions (such as fermentation temperature, pH, dissolved oxygen, inoculum size, etc.) to evaluate the growth curve, fermentation product yield, conversion rate, and production intensity of improved strains, providing a comprehensive assessment of their fermentation performance.
Fermentation Condition Optimization: Based on the fermentation performance evaluation results, we use statistical methods (such as response surface methodology, Plackett-Burman design, etc.) and experimental optimization techniques to optimize fermentation process parameters, enabling the strains to perform at their maximum production potential under optimal fermentation conditions.
Strain Improvement Technical Consulting and Customization
Technical Consulting Services: We offer professional consulting services for technical issues encountered during strain improvement, including strain improvement program design, technical problem-solving, and fermentation process improvement, helping clients develop reasonable strain improvement strategies.
Customized Strain Improvement Services: We provide customized strain improvement service plans based on the specific needs and goals of different clients. From strain selection to the final application of the improved strain, we offer one-on-one personalized services throughout the entire process to meet the client's needs in special fermentation product development and production.
Strain Improvement Services: From Start to Finish
1. Requirement Communication
Clients share their strain improvement needs. Our experts discuss production goals, raw materials, and technical challenges to propose practical solutions.
2. Project Evaluation
We assess project feasibility and define the technical approach. A detailed plan with timelines, milestones, and budget is provided.
3. Strain Screening
We select promising strains from our library or based on client input, then analyze their characteristics to evaluate improvement potential.
4. Gene Editing and Modification
Using CRISPR or similar tools, we precisely edit target genes to enhance metabolic pathways. We also confirm genetic stability of modified strains.
5. High-Throughput Screening
We use automated platforms to screen for strains with improved performance—based on criteria like yield, conversion efficiency, or stress tolerance.
6. Fermentation Optimization
We fine-tune fermentation parameters (e.g., pH, temperature, nutrients) using design of experiments (DOE) to maximize strain productivity.
7. Small-Scale Validation
We test the optimized strains in small-scale settings to verify performance and consistency, making adjustments as needed before scale-up.
8. Project Delivery
Validated strains are delivered with full technical documentation and training, enabling clients to independently scale and produce with confidence.
Strain Improvement Services: Why Choose Us?
Expert Team: Our skilled team of microbiologists, geneticists, and fermentation engineers delivers precise and efficient strain improvement services.
Cutting-Edge Technology: We use advanced gene editing, screening, and fermentation tools to enhance strain performance and boost productivity.
Customized Solutions: We tailor every project to the client's specific needs, ensuring practical and effective results for diverse applications.
Quality Assurance: Strict quality control ensures stable, consistent, and high-quality strains that meet global standards.
Ongoing Support: We offer continuous technical support to help clients apply strains successfully in real-world production.
Cost-Effective Results: Improved strains lead to higher yields, better quality, and lower costs—maximizing value and competitiveness.
FAQs
1. What are the main technological principles behind strain improvement services?
The primary technology used is gene editing, which involves precisely editing the target genes of microbial strains, including knockout, knock-in, and replacement operations, to alter their genetic traits and improve strain performance. Additionally, traditional breeding methods, such as ultraviolet (UV) light and chemical mutagens, may also be employed to induce mutations, followed by the selection of superior strains.
2. How do you ensure that the improved strains maintain stability and consistency?
During the strain improvement process, we strictly adhere to scientific operational procedures and quality control standards, ensuring tight quality control at every step. We conduct genetic stability analysis to ensure that the gene edits remain stable during the strain's growth and reproduction. Furthermore, during small-scale validation, we perform multiple tests and evaluations on the strain's growth characteristics and product generation to verify its stability and consistency.
3. If issues arise with the improved strains during practical application, does your company provide subsequent support?
Yes, we place great importance on customer experience and satisfaction. After project delivery, we provide a period of free technical support and after-sales services. If customers encounter any issues during use, our professional team will respond promptly and assist in resolving the problem, ensuring that customers can successfully apply the improved strains in their production processes.
