Submerged Fermentation (SmF)

Submerged Fermentation (SmF)

BOC Sciences has invented microbial fermentation platforms, including strain development, fermentation process optimization, and GARS services. These platforms are dedicated to providing our customers with high-quality products, customized services, and professional consulting for microbial fermentation projects.

BOC Sciences supports the industrial manufacturing of various biomolecules based on specialized fermentation knowledge and extensive fermentation facilities. Submerged fermentation is identified in which microorganisms are cultured in a liquid medium and subjected to continuous, vigorous agitation. BOC Sciences has expertise in submerged fermentation and provides customized products and quality fermentation services using submerged fermentation.

Introduction of Submerged Fermentation (SmF)

Submerged fermentation (SmF), also known as submerged liquid fermentation and liquid state fermentation, has been widely used to produce a range of metabolites. In this particular fermentation process, microorganisms are submerged in a solution containing nutrients required for growth. The choice of carbon and nitrogen sources includes monosaccharides, disaccharides, polysaccharides, cereals, legumes, bran, peptone, etc. Submerged fermenters can be classified as aerated or anaerobic and batch or continuous. The most common type of submerged fermentation involves agitation-aeration.

It is well known that submerged fermentation has been widely used for enzyme production. Since most industrial enzymes are secreted by cells into the external environment and remain in fermentation broth after the removal of biomass, thus enzyme purification can be carried out in a simple manner. The product is obtained directly from the fermenter without the need for cell disruption operations, and recovery of the desired molecules is achieved by filtration, centrifugation, or chromatography purification of the product.

Methods of Submerged Fermentation (SmF)

  • Continuous fermentation: Liquid nutrients are slowly and continuously added to the bioreactor at the same rate that the converted nutrient solution is recycled from the system, allowing the fermentation broth to maintain a steady rate of production.
  • Batch-fed fermentation: Growth nutrients are added to the fermenter during the cell culture process. This method facilitates higher cell density in the bioreactor, which is usually concentrated on simplifying product recovery. In addition, the risk of overflow metabolism is reduced by adding nutrients to maintain the growth rate of culture.

Advantages of Submerged Fermentation (SmF)

  • Wide range of substrates: Submerged fermentation can use for industrial starch, soybean meal, bran, and other low-cost industrial and agricultural products, as well as corn deep processing wastewater and starch wastewater as substrates.
  • Short production cycle: It is able to achieve optimal culture conditions by controlling aeration and agitation, temperature, pH, and medium fed in the bioreactor and executing the production of bioactive secondary metabolites in a shorter time period.
  • Various fermented products: In the process of submerged fermentation, a variety of bioactive molecules can be produced from the fermentation broth, including peptides, nucleic acids, amino acids, polysaccharides, enzymes, terpenoids, and sterols.

Applications of Submerged Fermentation (SmF)

  • Submerged fermentation process can be used for industrial-scale manufacturing of products such as citric acid, glycerol, or other valuable metabolites that can be processed into nutritional products, food additives, beverages, etc.
  • Submerged fermentation has been widely used for the production of industrial enzymes. It commonly applies bacteria and fungi to produce protein biomass of single-cell proteins.

What Can We Do?

As a premium fermentation service provider, BOC Sciences' ultimate goal is to help our customers accelerate the commercialization of their projects at the lowest cost possible. With our large commercial scale fermenters, we can achieve industrial production of target compounds through submerged fermentation. Moreover, our submerged fermentation technique can afford a wide range of bioactive substances, including enzymes, amino acids, polysaccharides, peptides, alkaloids, terpenoids, sterols and more.

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