Hydrolyzed Fumonisin B2
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| Category | Mycotoxins |
| Catalog number | BBF-05787 |
| CAS | 147985-10-2 |
| Molecular Weight | 389.61 |
| Molecular Formula | C22H47NO4 |
| Purity | ≥97% |
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Description
Hydrolyzed Fumonisin B2 is a metabolite of Fumonisin B2, a mycotoxin produced by mold associated with corn.
Specification
| Synonyms | Fumonisin AP2; Fumonisin HB2; Hydrolyzed Fumonisin B2; 3,5,14,15-Eicosanetetrol, 2-amino-12,16-dimethyl-, (2S,3S,5R,12S,14S,15R,16R)-; (2S,3S,5R,12S,14S,15R,16R)-2-Amino-12,16-dimethyl-3,5,14,15-eicosanetetrol |
| Storage | Store at -20°C |
| IUPAC Name | (2S,3S,5R,12S,14S,15R,16R)-2-amino-12,16-dimethylicosane-3,5,14,15-tetrol |
| Canonical SMILES | CCCCC(C)C(C(CC(C)CCCCCCC(CC(C(C)N)O)O)O)O |
| InChI | InChI=1S/C22H47NO4/c1-5-6-12-17(3)22(27)21(26)14-16(2)11-9-7-8-10-13-19(24)15-20(25)18(4)23/h16-22,24-27H,5-15,23H2,1-4H3/t16-,17+,18-,19+,20-,21-,22+/m0/s1 |
| InChI Key | BLYFGSXVLITXCG-FAGWYDQESA-N |
Properties
| Appearance | Brown to Yellow Film |
| Boiling Point | 551.7±40.0°C (Predicted) |
| Density | 1.004±0.06 g/cm3 (Predicted) |
| Solubility | Soluble in Methanol |
Reference Reading
1. Induction of apoptosis in cultured human proximal tubule cells by fumonisins and fumonisin metabolites
W Seefelder, H-U Humpf, G Schwerdt, R Freudinger, M Gekle Toxicol Appl Pharmacol. 2003 Oct 15;192(2):146-53. doi: 10.1016/s0041-008x(03)00262-x.
Fumonisin B1 (FB1) causes apoptosis in a variety of cell types and tissues but the apoptotic potential of other fumonisins and fumonisin metabolites has not been determined and the underlying mechanisms are not completely understood. In our studies we exposed human proximal tubule-derived cells (IHKE cells) to FB1, fumonisin B2 (FB2), fumonisin B3 (FB3), hydrolyzed fumonisin B1 (HFB1) and N-palmitoyl-hydrolyzed fumonisin B1 (N-Pal-HFB1) and investigated caspase-3 activation, chromatin condensation and DNA fragmentation. Exposure to 10 micromol/L FB1 for 24 h led to a significant increase in caspase-3 activity, chromatin condensation and to DNA fragmentation. All other tested compounds did not show any significant activation of caspase-3 activity nor chromatin condensation and DNA-fragmentation. Furthermore, we examined if a sphinganine accumulation is correlated with an induction of apoptosis in IHKE cells. Therefore we used a liquid chromatography/electrospray ionization-mass spectrometry(LC/ESI-MS)-method using phytosphingosine as an internal standard to determine sphinganine and sphingosine concentrations in IHKE cells. Whereas a significant increase of sphinganine (up to 7000% compared to control cells) was observed with all fumonisin-derivates, sphingosine levels nearly remained unchanged indicating that all substrates inhibited ceramide synthase effectively. These results demonstrate that all compounds let to increased sphinganine levels in IHKE cells but only FB1 was able to induce apoptosis. We conclude that the inhibition of the ceramide synthase is not per se a predictor whether or not fumonisins induce apoptosis.
2. Hydrolyzed fumonisins HFB1 and HFB2 are acylated in vitro and in vivo by ceramide synthase to form cytotoxic N-acyl-metabolites
Michaela Seiferlein, Hans-Ulrich Humpf, Kenneth A Voss, M Cameron Sullards, Jeremy C Allegood, Elaine Wang, Alfred H Merrill Jr Mol Nutr Food Res. 2007 Sep;51(9):1120-30. doi: 10.1002/mnfr.200700118.
Fumonisins B1 and B2 (FB1 and FB2) are the most abundant members of the fumonisins--mycotoxins that are produced by Fusarium verticillioides and are natural inhibitors of ceramide synthase. Their hydrolyzed forms, HFB1 and HFB2 (also called AP1 and AP2) are found in some foods, and they are not only inhibitors of ceramide synthase but also undergo acylation by this enzyme. This study characterized the conversion of HFB1 and HFB2 by ceramide synthase to their respective N-acylated metabolites using rat liver microsomes and palmitoyl-CoA or nervonoyl-CoA as cosubstrates, and examined animals that had been dosed with hydrolyzed fumonisins to ascertain if acylation occurs in vivo. Using an HPLC-MS/MS method that allowed the sensitive and selective detection of the acylation products, both HFB1 and HFB2 were found to be metabolized in vitro to nervonoyl- or palmitoyl-HFB1 and -HFB2 (i.e. C24:1-HFB1/2 and C16-HFB1/2, respectively). The apparent vmax was considerably higher for formation of C24:1HFB1 (157 pmol/min/mg protein) than for formation of C16HFB1 (8.7 pmol/min/mg protein). The acylation products also inhibited ceramide synthase and significantly reduced the number of viable cells in an in vitro [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay using a human colonic cell line (HT29). Furthermore, HPLC-MS/MS analysis of tissues from rats given intraperitoneal doses of HFB1 confirmed that formation of N-acyl-HFB1 occurs in vivo to produce metabolites with fatty acids of various chain lengths. The contribution of acylated HFB1 and HFB2 metabolites to fumonisin toxicity in vivo warrants further investigation.
3. Reduction of fumonisin B₁ in corn grits by twin-screw extrusion
Lauren S Jackson, Joseph Jablonski, Lloyd B Bullerman, Andreia Bianchini, Milford A Hanna, Kenneth A Voss, April D Hollub, Dojin Ryu J Food Sci. 2011 Aug;76(6):T150-5. doi: 10.1111/j.1750-3841.2011.02231.x. Epub 2011 Jun 21.
This study was designed to investigate the fate of fumonisins in flaking corn grits during twin-screw extrusion by measuring fumonisin B₁ (FB₁) and its analogs with a mass balance approach. Food grade corn grits and 2 batches of grits contaminated with FB₁ at 10 and 50 μg/g by Fusarium verticillioides M-2552 were processed with or without glucose supplementation (10%, w/w) with a twin-screw extruder. Extrusion reduced FB₁ in contaminated grits by 64% to 72% without glucose and 89% to 94% with added glucose. In addition, extrusion alone resulted in 26% to 73% reduction in the levels of fumonisin B₂ and fumonisin B₃, while levels of both mycotoxins were reduced by >89% in extruded corn grits containing 10% glucose. Mass balance analysis showed that 38% to 46% of the FB₁ species detected in corn extruded with glucose was N-(deoxy-D-fructos-1-yl)-FB₁, while 23% to 37% of FB₁ species detected in extruded corn grits with and without added glucose was bound to the matrix. It was also found that the hydrolyzed form of FB₁ was a minor species in extruded corn grits with or without added glucose, representing <15% of the total FB₁ species present. Less than 46% of FB₁ originally present in corn grits could be detected in the fumonisin analogues measured in this study. Research is needed to identify the reaction products resulting from extrusion processing of fumonisin-contaminated corn products. Practical application: Twin-screw extrusion is widely used in food industry for its versatility. This technology may reduce the level of fumonisins in corn particularly with added glucose.
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Bio Calculators
* Our calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
It is commonly abbreviated as: C1V1 = C2V2
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g/mol
Tip: Chemical formula is case sensitive. C22H30N4O √ c22h30n40 ╳
