Acremonium chrysogenum Fermentation Services

BOC Sciences is a leading CDMO capable of supplying high quality metabolites. We offer Acremonium chrysogenum fermentation services to perform fermentation production of antibiotics. Through our team of experts in bioengineering and organic chemistry, we are able to combine fermentation and synthesis approaches to provide cephalosporin C and its derivatives to our customers at competitive prices.

Introduction of A. chrysogenum Fermentation

Acremonium chrysogenum, abbreviated as A. chrysogenum, is an aerobic filamentous fungus with a complex cell wall structure and a specific life cycle. A. chrysogenum is a major strain for the industrial production of a β-lactam antibiotic, cephalosporin C (CPC). Although few filamentous fungi are used in the industrial production of antibiotics, the production of cephalosporins by submerged fermentation using A. chrysogenum is still the main choice. However, cephalosporin production by A. chrysogenum suffers from low yields and titers compared to penicillin production by Penicillium chrysogenum, both penicillin and cephalosporin are β-lactam antibiotics.

Biosynthesis of Cephalosporin by A. chrysogenum

Given the limitations of wild A. chrysogenum strains, genetic engineering modification and metabolic engineering regulation of A. chrysogenum are important approaches to manipulate A. chrysogenum to obtain high-yielding mutant strains. two gene clusters on the chromosome of A. chrysogenum are involved in the biosynthesis of CPC. These gene clusters have been identified and intensively studied and they consist of pcbAB-pcbC, cefD1-cefD2, and cefEF and cefG, which encode enzymes that play different roles involved in CPC biosynthesis, respectively. The introduction of exogenous genes into A. chrysogenum strains, high-throughput screening to obtain high-performance strains, and real-time detection and control during fermentation are important ways to improve the production of CPC.

Applications of A. chrysogenum Fermentation

A. chrysogenum is an important industrial microorganism, and its fermented production of cephalosporin C is an important resource for the industrial manufacture of many cephalosporin-based antibiotics. The structural cores of many important cephalosporin compounds are derived from CPC. These antibiotics are semisynthetic derivatives of CPC with a unique cephem nucleus that includes a β-lactam ring bound to a 6-membered sulfur containing dihydrothiazine ring. Therefore, the fermentation production and cost of CPC is critical to the cephalosporin antibiotics market.

Our Services for A. chrysogenum Fermentation

Strain improvement and selection

DNA manipulation and metabolic engineering of A. chrysogenum strains involving molecular biology methods including plasmid transformation, PCR, Northern and Southern blot analysis, and enzyme activity assays.

Fermentation process optimization

A. chrysogenum is selected as the host strain for fermentation process optimization to explore the optimal fermentation conditions such as medium composition, pH, temperature, to maximize the yield of target compounds. In addition, key bioprocess variables such as biomass, substrate and product concentrations can be monitored online during the fermentation process.

Isolation, purification and characterization

After fermentation, the biomass is processed, and the target products can be separated and purified by filtration, centrifugation, and chromatography. The purity and yield of the target product can be tested by UPLC/MS.

BOC Sciences' A. chrysogenum Fermentation

A. chrysogenum fermentation production
Patented and proprietary manufacturing methods
State-of-the-art facilities and aseptic products
Total fermentation capacity of over 2,000,000 liters
Complete quality management system with ISO 9001 certification